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Whole Lung Quantitative Analysis of MicroRNAs by Using Single-Cell Sequencing.
Lung cancer is the most common form of cancer and is responsible for the highest cancer-related mortality worldwide. Identifying the biogenesis of microRNAs, especially in nonmalignant cells, can help to develop novel treatments. Here, we describe a method of quantitative analysis of microRNAs in one or more whole human lung samples that can identify changes in the miRNA profile caused by the introduction of a specific therapeutic. This method will allow us to identify microRNAs that are secreted by tumor cells and can function as tumor-suppressive or oncogenic factors as they diffuse in the tissue. The method can be used to identify the biogenesis of the microRNAs within the tissue as well as the changes in these microRNAs caused by the introduction of a therapeutic. The single-cell RNA sequencing (scRNA-seq) methodology described here is already used for clinical diagnostics and provides a platform for identifying the exact changes in the microRNA expression caused by the introduction of a therapeutic.The present invention relates generally to the field of data processing, and more particularly to the field of intelligent system-on-a-chip (SoC) technologies.
SoC devices are increasingly being used to provide an intelligent signal processing core for applications such as sensing and control applications. In order to provide convenient power connections and flexible interface connectivity, chip designers will typically build in power distribution circuitry and an interface in the same integrated circuit package. However, since power consumption is often a major contributor to total system cost, the overall power efficiency and cost of the system can be significantly improved.
By way of example, FIG. 1 shows a prior art system 10 that includes an SoC unit 12 with a core 14 and a power supply 16. The core 14 is in communication with an external bus interface 18. In order to interface to the external bus interface 18, the core 14 is in communication with a higher-level interface circuit 20 that includes an

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