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Pseudomonas syringae pv. glycinea is the causal agent of green peach aphid (GPA) green pitting. This bacterium produces the ice nucleation active factor (INF), a heat-stable proteinaceous compound that is important for biofilm formation, and which is essential for GPA-GPA interaction. In this study, a real-time PCR was developed and validated to detect the presence of the INF gene in P. syringae pv. glycinea. The assay was tested using a collection of 69 isolates from several geographic origins and host species. A melting curve was acquired at the end of the PCR-program, and a single melting temperature (Tm) of 87.5±0.2°C was determined. The probe was designed to be complementary to the 3′ region of the INF gene to avoid interference with other genes located in the same region. The specificity of the PCR assay was confirmed by melt curve analysis. The detection limit was 100 copies/ml, and the sensitivity was tested with different concentrations of the target plasmid, confirming a logarithmic relationship between the concentration of the target and the threshold cycle.Over 200 companies and organizations from around the world are calling on the Federal Communications Commission to avoid taking any action at this week
BollywoodConventionally, an image processing apparatus is known in which an IP (Internet Protocol) camera is connected to a server via a network and the respective channels of the IP camera and the server are operated by an application program.
Moreover, the CPU (Central Processing Unit) of the image processing apparatus processes image data according to the settings (resolution and frame-rate, for example) of the camera and the settings (exposure time, white balance, brightness and color and the like) of the internal or external light sources (lighting devices) to generate and output an image. This image processing method is called a “console method” (so-called “pull method”).
In another method, the CPU of the image processing apparatus acquires the settings (resolution and frame-rate, for example) of the camera and the settings (exposure time, white balance, brightness and color and the like) of the internal or external light sources from the server via the network. This image processing method is called a “push method” (so-called “pushing method”).
In the pull method, image processing is performed on an image captured by the IP camera. However, to acquire the settings of the camera in the pull method, it is necessary to read the settings of the camera via the network. This requires the settings of the camera to be saved in advance in the server. This also requires setting of the IP camera (camera body) each time the settings of the camera are changed.
In contrast, in the push method, image processing is performed on an image captured by the IP camera. This processing is initiated when the settings of the camera are first changed.
For example, when the IP camera is added, setting of the camera is performed. In this case, the settings of the camera are acquired from the server.
The settings of the IP camera are saved in a database, for example. When image processing is performed, the settings of the IP camera and an image captured by the IP camera are checked. The settings of the IP camera are acquired from the database, and the acquired settings are set in the IP camera.
Furthermore, when the settings of the IP camera are changed, it is necessary to transmit an image captured by the IP camera to the server, and the server acquires the settings of the IP camera from the database.
The settings of the IP camera can be varied for each camera. Specifically, the sizes of the IP camera and
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